8 - HYDROGEN GAS INHALATION IMPROVE CORONARY ARTERY DILATION IN A MICE MODEL OF KAWASAKI DISEASE

Kawasaki Disease (KD) is a syndrome primarily affecting young children, typically under the age of five and is characterized by the development of acute vasculitis. Through extensive research conducted on both murine and human subjects, it has been demonstrated that the heightened levels of reactive oxygen species (ROS) play a pivotal role in the development of KD especial the coronary artery lesions (CAL). Hydrogen gas exhibits potent antioxidant properties that effectively regulate ROS production and the inflammatory response. In the current study, we used lactobacillus casei cell wall extract (LCWE)-induced vasculitis in mice as an animal model of KD and treated by hydrogen gas inhalation for developing therapeutic strategies for KD. We observed significant dilatation and higher Z score of LCA in D21 and D28 in mice after LCWE treatment compared to the control group (p < 0.05) and significant resolution of LCA diameter (p < 0.005) and Z score (p < 0.005) after treatment with inhaled hydrogen gas. We further demonstrated that higher serum IL-6 expression in mice after LCWE treatment (p < 0.005) and IL-6 significantly decreased after inhaled hydrogen gas therapy (p < 0.0005). In conclusion, from literature review this is the first report that hydrogen gas inhalation demonstrated effective prevent coronary artery dilatation in the KD murin model.